D7291

Sigma

 

Deoxyribonuclease I RNase-free solution from bovine pancreas

buffered aqueous glycerol solution, ≥10,000 units/mg protein

CAS Number:9003-98-9
Enzyme Commission (EC) Number:3.1.21.1   ( BRENDA | IUBMB )
EC Number:232-667-0
MDL number:MFCD00130918

Description

Biochem/physiol ActionsDigests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5' phosphates and 3' OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Features and Benefits• Prepare DNA for nick translation.1
• Footprinting assays to determine DNA-protein interactions.2
• RNA purification by removing DNA.
Unit DefinitionOne unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
Physical formSolution in 50% (v/v) glycerol, 20 mM sodium acetate, pH 6.5, 5 mM CaCl2, 0.1 mM PMSF

Properties

formbuffered aqueous glycerol solution
shipped indry ice
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Safety Statements22-24/25
WGK Germany3
RTECSRF0750000

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References

Cited Reference1. Meinkoth, J. and Wahl, G. M., Nick translation. Meth. Enzymol. 152, 91-94, (1987) Abstract
 2. Galas D. J., DNase footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res. 5, 3157, (1978) Abstract